The techniques which have been used for the fecal examination of ruminant infected with the pancreatic flukes, Eurytrema Pancreaticum, were reviewer in their efficiency to detect the ova.
One of modified fecal examination: H.F.E. (hydrochloric acid-formalin-ether) sedimentation method was devised in this study.
Efficiency in the detecting ability of the fluke eggs with H.F.E. sedimentation method was determined by a series of repeat tests. Among 20 head of cattle known to harbor 1-5 adult worms of the pancreatic fluke, 75% of the infected cattle were detected, and among 60 head of cattle known to harbor more than 6 adult worms, 95% of the infected cattle were detected with H.F.E. sedimentation method .
The procedures of the H.F.E. sedimentation method are as follows;
1) Take the sample 5-10 gm., emulsify throughly with 20 ㎖. of 50% hydrochloric acid in a cup.
2) Strain this mixture through one or two layers of wet surgical gauze into 15 ㎖. centrifuge tube.
3) Washing the cup with 5 ㎖. of 50% hydrochloric acid and strain again.
4) Centrifuge at 2,300 rpm. for 2 minutes.
5) Pour off the supernatant fluid.
6) After the sediment mixed with 10% formalin, stand for 5 minutes.
7) Add 2-3 ㎖. of ether, shake vigorously up and down, after the top of the tube covered with thumb.
8) Centrifuge at 2,300 rpm. for 2 minutes.
9) Loosen the fecal plug in the tube by ringing with an applicator stick.
10) Quickly, but carefully, pour of all, but the bottom layer of sediment.
11) Thoroughly mix the sediment, pour on a slide (or pick up it with a pipett), mount with a cover glass.
12) Examine carefully.
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